Abstract
High resolution two-dimensional 1H and 13C nuclear magnetic resonance at 500 MHz was used in combination with molecular modelling to solve the structures of the antigenic O-polysaccharides produced by Escherichiahermannii strains ATCC 33650 and 33652. Classical structural methods such as methylation analysis, selective and partial hydrolysis, and periodate oxidations confirmed that the O-polysaccharides had a branched tetrasaccharide repeating unit with the structure:[Formula: see text]Keywords: Escherichiahermannii, lipopolysaccharide, magnetic resonance, polysaccharide.
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