Abstract
The repeating unit of the acidic polysaccharide secreted by Rhizobium trifolii Bart A, a non-nodulating strain, is an octasaccharide containing O-acetyl and pyruvic acetal groups. The polysaccharide contains d-glucose, d-galactose, d-glucuronic acid, pyruvic acid, and acetic acid in the molar ratios 5:1:2:2:3. The glycosyl residues are all pyranoid and only the linkages between the repeating units are α. The repeating unit is different from that found in another non-nodulating strain but the same as that found in a nodulating strain of the same bacterial species, and the following structure is proposed.
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