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Abstract
We have analyzed derivatives of the plasmid R1drd19 carrying the transposon Tn10 by electron microscopy following denaturation and renaturation of the molecules, and by digestion with various restriction enzymes, gel electrophoresis and Southern blotting. We show: 1) that the published restriction map of R1drd19 is inconsistent with our results. We present a modified map which is consistent with our data. 2) that R1drd19 carries a single resident copy of the element IS10 which is normally associated with Tn10 as an inverted repeat, and 3) that R1drd19 carries three copies of the insertion element IS1 in the resistance determinant region.
Highlights
The plasmid Rldrd 1 9 (Meynell and Datta 1967) is closely related to the multiple antibiotic resistance plasmids R6 and R100.1
2) that Rldrd19 carries a single resident copy of the element ISIO which is normally associated with TnlO as an inverted repeat, and
Heteroduplex molecules formed between Rldrd 1 9 and R100.1 (Sharp et al 1973 and Fig 7a) show three major differences in structure~ One is associated with the insertion of the tetracychne resistance transposon TnlO into the RTF region of RI00.1 ; two are located in the r-determinant
Summary
The plasmid Rldrd 1 9 (Meynell and Datta 1967) is closely related to the multiple antibiotic resistance plasmids R6 and R100.1. It has considerable homology with these plasmids (Sharp et al 1973) and is composed, in a similar way~ of a cluster of drug resistance genes (r-determinant or r-det) flanked by two directly repeated I S / elements Heteroduplex molecules formed between Rldrd 1 9 and R100.1 (Sharp et al 1973 and Fig 7a) show three major differences in structure~ One is associated with the insertion of the tetracychne resistance transposon TnlO into the RTF region of RI00.1 ; two are located in the r-determinant. The two latter occur at the extremity proximal to the origin of vegetative replication and involve the insertion into Rldrdl of the ampicillin resistance transposon Tn3 and of a 9.6 kilobase (kb) segment carrying resistance to kanamycin (Kmr).
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