Abstract
Phaeocystispouchetii virus (PpV0l), first isolated from Norwegian coastal water in 1995, infects and lyses the haptophyte Phaeocystis pouchetii Lagerheim. PpV0l has not yet been assigned to a family. Phaeocystis may play an important role in ocean climate control and PpV0l infection could alter the amount of this haptophyte. The virion contains a dsDNA genome (∼450 kbps) encapsulated within a large icosahedral shell. The most abundant protein has a mass of 59 kDa. More than five minor polypeptides with masses ranging from 30 to 50 kDa have also been identified. We have used cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction methods to examine the native morphology of PpV0l.Purified PpV0l samples were prepared for Cryo-EM as described. Images were recorded on film at 33,000x nominal magnification in a Philips CM300 FEG microscope at 300kV, at 1.0μm to 2.0μm under focus, and with an electron dose of 2200e-/nm2 (Fig. 1A).
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