Abstract
A physical map of the genome of cauliflower mosaic virus (CaMV) has been constructed on the basis of the cleavage of CaMV-DNA by restriction endonucleases. Digestion of circular native viral DNA (1) with Eco RI endonuclease produces three fragments, (2) with Hin dIII endonuclease produces nine fragments, and (3) with Bam HI endonuclease produces three fragments from the average virus DNA molecule in the population. The restriction fragments have been ordered by examining the overlapping fragments and the partial digestion products generated by these three restriction endonucleases. Restriction fragments of the virus DNA generated by Eco RI and Bam HI endonucleases have been molecularly cloned in bacterial plasmids in Escherichia coli in order to facilitate restriction mapping of the virus DNA. Evidence for nucleotide sequence heterogeneity in the native virus DNA population is apparent in the form of uncommon restriction endonuclease sites found in only a small portion of the DNA molecules. Examination of molecularly cloned fragments of virus DNA containing these variable sites allowed a more complete analysis of this phenomenon.
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