The Structure and Hormone Expression of Male Accessory Reproductive Glands of the Sunda Porcupine (Hystrix Javanica)

Linda Miftakhul Khasanah ,Teguh Budipitojo ,Irma Padeta ,Synthia Regita Noor Mahesty

doi:10.7176/alst/78-04

Abstract

This research was to investigate the histological structure of male accessory reproductive glands of the Sunda porcupine and verify the presence of testosterone and Androgen Binding Protein (ABP) with immunolocalization. The research used three accessory reproductive gland samples of male Sunda porcupine. Tissues were processed using paraffin method and stained with Hematoxylin-Eosin and Immunohistochemistry methods. The histological structure and distribution of testosterone and ABP were analyzed descriptively. This research showed that male accessory reproductive glands of the Sunda porcupine are seminal vesicle, prostate, and bulbourethral glands. The seminal vesicle was lined by simple columnar epithelium, prostate was lined by simple columnar epithelium or simple cuboidal epithelium, and bulbourethral gland was lined by simple cuboidal epithelium. The presence of testosterone was found in adenomere cells of seminal vesicle and prostate glands; and in interstitial tissue of bulbourethral gland. The presence of ABP was detected in the adenomere cells of the bulbourethral gland. The male accessory reproductive glands of Sunda porcupine has been identified which consist of seminal vesicle, prostate, and bulbourethral glands. The immunolocalization of testosterone was found in adenomere cells of seminal vesicle and prostate glands, and ABP was detected in bulbourethral gland. Keywords: Androgen binding protein, Bulbourethral, Hystrix javanica , Prostate, Seminal vesicle, Testosterone DOI : 10.7176/ALST/78-04 Publication date: February 29 th 2020

Highlights

Sunda porcupine is classified in the Kingdom of Animalia, Filum Chordata, Mammalia class, Order Rodentia, Family Hystricidae, Genus Hystrix, and Species Hystrix javanica (Duff & Lawson 2004)
The prostate gland is a branched tubuloacinar gland located between the urethra and the seminal portion of the ventral section (Fig. 2), while the bulbourethral gland is a mixed tubuloacinar gland located outside the pelvic cavity next to the caudal the prostate-looting is covered by the musculus ischiocavernosus
The results of this study indicate that the male accessories glands of Sunda porcupine (Hystrix javanica) consists of seminal vesicles gland, prostate gland and bulbourethral gland

Summary

Introduction

Sunda porcupine is classified in the Kingdom of Animalia, Filum Chordata, Mammalia class, Order Rodentia, Family Hystricidae, Genus Hystrix, and Species Hystrix javanica (Duff & Lawson 2004). According to The International Union for Conservation of Nature (IUCN) of conservation status of Sunda porcupine is included in the category least concern (Lunde & Aplin 2008). The reproductive system in males is composed of testicles, epididymis, genital accessories glands, duct deferens, urethra, and penis. Plasma seminal is an assortment of fluid produced by the prostate gland, the seminal vesicle gland, and the bulbourethral molar that will be poured into the urethra and will mix with spermatozoa fluid and secretion of ampullary gland, duct deferens, as well as liquids derived from epididymis

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