The structure and function of the adhesive organ in strigeid trematodes

Abstract

The biology and life-cycle of Holostephanus lühei is briefly described. The similarities in morphology between the adhesive organ gland cells of H. lühei and Cyathocotyle bushiensis are stressed. Alkaline and acid phosphatases were demonstrated in the adhesive organ gland cells and cuticle. Acid phosphatase also occurs in the caecal cells. Non-specific esterase, sensitive to E600 10−5M and Mipafox 10−3M, is present in the gland cells. The caeca contain a non-specific esterase sensitive to AgNO3 10−2M and PCMB 10−4M. Leucine aminopeptidase is present in the gland cells. The in vitro studies confirmed that the secretory products pass to the exterior of the parasite and have a histolytic action.The four strigeids studied, Cyathocotyle bushiensis, Holostephanus lühei, Diplostomum spathaceum and Apatemon gracilis minor, are compared and the biological role of the secreted enzymes are discussed.My sincere thanks are due to Dr D. A. Erasmus for suggesting this line of investigation and for his encouragement during the study. I am also grateful for his performing, on my behalf, the infection experiments and criticizing the manuscript. I wish to thank Professor J. Brough for his interest in the progress of the work, and the University College, Cardiff, for a grant from William E. Morgan's Bequest.