Abstract
The large RNA component (70 S) of murine leukemia virus (MLV) is irreversibly converted to 38 S subunits by treatment with dimethyl sulfoxide (DMSO). According to sedimentation and gel electrophoretic analyses, the 38 S subunits of newly synthesized MLV are homogeneous in size with a molecular weight of 3.6 to 4.0 × 10 6 daltons. However, RNA subunits of virions that have been incubated at 37° for 3–6 hours are composed of a heterogeneous assortment of sizes, probably arising from the RNA degradative activity that can be detected in virions. The 70 S RNA structure found in MLV cannot be detected in MLV-infected cells. However, when cytoplasmic RNA is first treated with DMSO to convert to the 38 S form the viral RNA that is normally assembled into the 70 S structure, then RNA with an electrophoretic mobility and base composition similar to that of 38 S subunit RNA can be isolated. This cytoplasmic 38 S component amounts to 0.1–0.4% of the total cytoplasmic RNA. The 70 S RNA component found in MLV may be formed during virion assembly and release from the cell.
Published Version
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