The stress effect of atrazine on the inducible defense traits of Daphnia pulex in response to fish predation risk: Evidences from morphology, life history traits, and expression of the defense-related genes

Abstract

Herbicide pollution is persistent, which not only has a negative impact on individual organisms, but also may endanger the interspecific relationship between predators and prey. Cladocerans, i.e. zooplankton that plays an important role in the energy flow and material circulation in freshwater ecosystem, usually develop induced defense in response to predation risk. We used atrazine, one of the most used herbicides in the world, and Daphnia pulex, a representative cladocerans, to test the possible interference effect of herbicides on the induced defensive traits of cladocerans in response to predator fish (Rhodeus ocellatus) kairomone, including morphological defense, life history strategies, and the expression of defense-related genes. Atrazine reduced the body size, spine size, growth rate, total offspring, and the relative reproductive output of D. pulex, which further affected the response strength of the morphological and life history defenses, i.e., atrazine significantly reduced the spine size, relative spine size, and fecundity of D. pulex in response to R. ocellatus kairomone. Exposure to atrazine affected the expression of defense-related genes, and we speculated that atrazine affected the signaling process in the induced anti-predation defense of cladocerans. Specially, fish kairomone attenuated the negative effects of high concentrations of atrazine on the life history traits of D. pulex. Our results will help to accurately assess the potential risk of artificial compounds in freshwater ecosystems from the perspective of interspecific relationships, and help to understand the impact of environmental changes on the inducible anti-predator defense of prey in aquatic ecosystems.