Abstract

Mao or Makmao ( Antidesma thwaitesianum Mull. Arg.) is a wild plant found in the northeast of Thailand. Mao is one of fruits which are source of anthocyanins. Mao fruits are used for juice and concentrate which are consumed for healthy drinks. Determination of the kinetic parameters is essential to predict the quality changes and stability of anthocyanins in Mao juice and concentrate that occur during storage. The purpose of this research was to study the degradation of anthocyanins in Mao juice and concentrate during storage at 5, 30 and 37°C for 15 days. The storage stability of Mao anthocyanins was studied in 15°Brix juice and 45°Brix concentrate. The degradation kinetic (k), half-life (t 1/2 ), activation energy (E a ) and Q 10 values for Mao anthocyanins degradation were determined. The results indicated that analysis of kinetic data for the degradation of anthocyanins followed a first-order reaction. An increase storage temperature from 5 to 30 and 37°C increased k value of anthocyanins in Mao juice and concentrate. Increasing storage temperature decreased t 1/2 value of anthocyanins in both concentrations. At 5, 30 and 37°C, the t 1/2 of anthocyanins decreased from 35 to 13 and 5 days for Mao juice and 32 to 25 and 21 days for Mao concentrate. The E a value of the anthocyanins degradation in Mao juice and concentrate were 38.03 and 8.42 kJ/mol, respectively. Q 10 values of both Mao juice and concentrate at 30-37°C were higher than those were storaged at 5-30°C. Thus higher stability of anthocyanins was achieved by using concentration and storage at lower temperature.

Highlights

  • Mao fruits are used for juice and concentrate which are consumed for healthy drinks and theirs have become more popular in Thailand

  • The results clearly indicated that anthocyanin degradation increased with increasing storage temperature and time

  • The results found that increasing anthocyanin degradation incresed with increasing storage temperature but t1/2 values decreased

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Summary

Introduction

Mao is one of fruits which are source of anthocyanins and antioxidants [1,2,3]. Mao fruits are used for juice and concentrate which are consumed for healthy drinks and theirs have become more popular in Thailand. Anthocyanins are a good source of natural antioxidant, in addition, anthocyanins have been reported to be highly effective in reducing cholesterol levels in the human body including preventing cardiovascular diseases and anticarcinogenic activity [4, 5]. Anthocyanins are natural pigments which are responsible for the attractive red, purple and blue colors in Mao fruits. Anthocyanin are rather unstable during processing and storage, which readily degrade and form colorless or undesirable brown-colored compounds [6, 7]. Temperature, time, solid content and storage conditions are considered to be important factors influencing its stability [8]

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