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Abstract
The presence of adenosine 3‘,5‘-monophosphate was demonstrated in bovine luteal tissue. The addition of luteinizing hormone to incubating slices of a bovine corpus luteum caused a rapid accumulation of this cyclic nucleotide which preceded the increase in progesterone synthesis. This effect appeared to be specific for luteinizing hormone. Puromycin did not inhibit this action of luteinizing hormone on cyclic AMP accumulation. In view of the previous finding that exogenous cyclic AMP added to incubating slices can mimic the effect of luteinizing hormone on steroidogenesis, these results indicate that the cyclic nucleotide is a mediator of this action of luteinizing hormone. An increase in the concentration of cyclic AMP has also been demonstrated in a human corpus luteum stimulated in vitro by human chorionic gonadotropin.
Highlights
Since adenosine 3’,5’monophosphate had been implicated in the steroidogenic effect of adrenocorticotropic hormone on the adrenal cortex [6], it seemed possible that the response of the corpus luteum to luteinizing hormone might be mediated by this cyclic nucleotide
Effect of Luteinizing Hormone on Cyclic AMP Concentration in Luteal S&es-After a prior incubation of 1 hour, paired slices of a corpus luteum were incubated in Krebs-Ringer bicarbonate medium alone or in the presence of luteinieing hormone (2.0 pg per ml)
This procedure did not produce any major or consistent change in the control levels of cyclic AMP, there was a slight enhancement of the effect of luteinizing hormone in the slices subjected to the l-hour prior incubation
Summary
The presence of adenosine 3’,5’-monophosphate was demonstrated in bovine luteal tissue. The addition of luteinizing hormone to incubating slices of a bovine corpus luteum caused a rapid accumulation of this cyclic nucleotide which preceded the increase in progesterone synthesis. This effect appeared to be specific for luteinizing hormone. Since adenosine 3’,5’monophosphate had been implicated in the steroidogenic effect of adrenocorticotropic hormone on the adrenal cortex [6], it seemed possible that the response of the corpus luteum to luteinizing hormone might be mediated by this cyclic nucleotide. The studies reported in this communication deal with the effects of luteinizing hormone on the endogenous cyclic AMP concentrations in bovine and human corpora lutea and the correlation between these levels and progesterone synthesis
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