The stimulating effect of ammonium on nodulation in Pisum sativum L. is not long lived once ammonium supply is discontinued

Abstract

Although mineral N generally has a negative effect on legume-rhizobia symbioses, experiments in hydroponic culture in our laboratory (Waterer et al., 1992) have shown that low concentrations of NH+4 can stimulate nodulation in pea (Pisum sativum L.). The objectives of the current study were to determine the immediate and residual effects of NH+4 on nodulation and N2 fixation in pea in sand culture. Peas (cv. Express) were exposed to 0.0, 0.5, 1.0, and 2.0 mM of 15N-labelled (NH4)2SO4 for 28 days after inoculation (DAI). From 28 to 56 DAI the plants were grown on a NH+4-free nutrient solution. Plants were harvested at 7, 14, 21, 28 and 56 DAI and nitrogenase activity was measured by gas exchange at 28 and 56 DAI. Root, shoot, and nodule dry weight (DW) and total N content were obtained, in addition to nodule counts and 15N enrichment of plant composites. The 1.0 and 2.0 mM NH+4 treatments consistently resulted in higher total plant DW accumulation than the control (0.0 mM NH+4). At 28 DAI, plants exposed to 1.0 and 2.0 mM NH+4 had 1.8 to 2.8 times more nodules plant-1, respectively, and plants exposed to 2.0 mM NH+4 had 1.7 fold higher specific nodulation (nodule number g-1 root DW). However, individual nodule DW was greater in control plants, such that there were no differences in nodule DW per plant among treatments. Ammonium treatment resulted in more nitrogen derived from the atmosphere (NDFA) in peas early in the experiment, but by 28 DAI there were no treatment effects on NDFA. Whole plant and nodule specific nitrogenase activity (µmol H2 g-1 nodule DW h-1) was higher in control plants at 28 DAI. However, by 56 DAI, after an additional 4 weeks of NH+4-free nutrition, no differences in nitrogenase activity nor whole plant or specific nodulation were detectable. This study indicates that nodulation in pea is stimulated in sand culture while exposed to NH+4. However, once NH+4 is removed, relative growth rate, nodulation and nitrogenase activity becomes similar to plants that were never exposed to NH+4.