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Abstract
The relationship between the induction of retinal glutamine synthetase in tissue culture and changes in the corticosteroid-binding capacity of serum in the culture medium was investigated. As measured by equilibrium dialysis, corticosteroid-binding capacity was consistently higher in the medium after the culture period indicating a removal of corticosteroid from the medium during this time. The effect of heat inactivation of serum corticosteroid-binding globulin and the effect of steroid depleted (stripped) serum in the medium on the induction were studied. The uptake of [1,2− 3H 2]-cortisol by retinal tissue in the presence of varying concentrations of stripped serum was found to parallel glutamine synthetase induction. These studies demonstrate: (1) the uptake of endogenous glucocorticoid of the serum-containing culture medium during glutamine synthetase induction; (2) the identity of steroid with a previously reported inducing factor in serum; and (3) an influence of corticosteroid-binding globulin in mediating the effects of the hormone.
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