Abstract
Exposure to magnetic field (MF) can affect cellular metabolism remotely. Cardio-toxic effects of Doxorubicin (DOXO) have limited clinical uses at high dose. MF due to its effect on reactive oxygen species (ROS) lifetime, may provide a suitable choice to boost the efficacy of this drug at low dose. Here, we investigated the potential effects of homogenous static magnetic field (SMF) on DOXO-induced toxicity and proliferation rate of cancer cells. The results indicated that SMF similar to DOXO decreased the cell viability as well as the proliferation rate of MCF-7 and HFF cells. Moreover, combination of 10 mT SMF and 0.1 µM DOXO decreased the viability and proliferation rate of cancer and normal cells in a synergetic manner. In spite of high a GSH level in cancer cell, SMF boosts the generation and lifetime of ROS at low dose of DOXO, and overcame to GSH mediated drug resistance. The results also confirmed that SMF exposure decreased 50% iron content of cells, which is attributed to iron homeostasis. In conclusion, these findings suggest that SMF can decrease required dose of chemotherapy drugs such as DOXO and thereby decrease their side effect.
Highlights
Doxorubicin (Adriamycin), Epirubicin (Ellence), Docetaxel (Taxotere) and Paclitaxel (Taxol) are among the most common types of chemotherapy drugs, which are currently used to treat breast cancer in women[10]
We aimed to examine the impact of continuous homogeneous static magnetic field (SMF) exposure on the facilitation of reactive oxygen species (ROS) (OH, O2−) generation and on iron content changes as well as its effect on DOXO performance in breast cancer cells
We assessed the effect of continuous SMF exposure on the viability of MCF-7 and human foreskin fibroblast (HFF) cells at different intensities (5, 10, 15 and 20 mT) for both exposure times (24 and 48 h)
Summary
Doxorubicin (Adriamycin), Epirubicin (Ellence), Docetaxel (Taxotere) and Paclitaxel (Taxol) are among the most common types of chemotherapy drugs, which are currently used to treat breast cancer in women[10]. Free radicals are the by-products of cellular metabolism They act as regulatory mediators in the regulation of cell homeostasis at low or moderate concentrations. Studies showed that SMF has more effects with chemotherapy drugs such as Adriamycin in the tumor cells[22,23]. It seems that treatments with multiple-agent as used in combination of physical (radiations, MF) and chemical (drugs) agents could increase the antitumor efficacy and decrease the drug-resistance in tumors[24]. We aimed to examine the impact of continuous homogeneous SMF exposure on the facilitation of ROS (OH, O2−) generation and on iron content changes as well as its effect on DOXO performance in breast cancer cells
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