THE STATE OF THE HEMOCOAGULATION LINK IN THE HEMOSTASIS SYSTEM OF MICE AFTER PARTIAL LIVER RESECTION UNDER ADMINISTRATION OF PLATELET AUTOMESOCONCENTRATE

Abstract

The purpose of the study is to evaluate the indicators of the hemocoagulation link in the hemostasis system of mice after partial resection of the liver under the platelet automesoconcentrate administration.
 Materials and methods. 2/3 of the liver was resected in wild-type mice. The animals were divided into three groups: I - control; II – mice that underwent partial hepatectomy; ІІІ – mice that were injected with automesoconcentrate of platelets in a dose of 1 ml/kg during surgery into the liver remnant.
 Results and discussion. The study has shown that during the first day following the liver resection, hemocoagulation processes decrease that was indicated by a reduction in the content of platelets and fibrinogen in the blood and a simultaneous increase in the parameters of thrombin time, prothrombin time, and International Normalized Ratio. In the period from the 4th to the 7th day of the study, an increase in the levels of platelets and fibrinogen and a decrease in the indicators of thrombin time, prothrombin time, and Activated Partial Thromboplastin Timer were revealed, however, the studied indicators did not acquire the values ​​of the control group of animals. Administration of automesoconcentrate is a dose of 1 ml/kg directly into the liver remnant during the hepatectomy procedure helps to normalize the hemocoagulation system on the seventh day after the procedure.
 Administration of automesoconcentrate also prevents the development of hypocoagulation, vascular wall defects (vasculitis, reduction in the thickness of the walls of microvessels), deficiency of blood coagulation factors. The high concentration of platelets in the automesoconcentrate enables to effectively avoid the loss of platelets and fibrinogen, coagulation factor V, coagulation factor VIII and other coagulation components. Along with this, the automesoconcentrate helps to restore the functional capacity of the liver after resection of a significant part of it, since hepatectomy damages the liver function of patients.
 Conclusions. Administering platelet automesoconcentrate effectively prevents the loss of platelets, fibrinogen, coagulation factor V, coagulation factor VIII, and other crucial coagulation components.