Abstract
INTRODUCTION: The microbial community of the colon is a single microecological system which quickly responds to the exposure to external and internal factors with quantitative and qualitative changes with the result of development of the condition termed dysbiosis. The changes in the composition of colon microbiocenosis can lead to a number of diseases and to the development of immunopathological and allergic conditions.
 AIM: To evaluate the state of the colon microbiocenosis and antioxidant properties of rats’ colonocytes against a background environmental dysbiosis and the use of probiotic Bifidumbacterin® and symbiotic Acipol®.
 MATERIALS AND METHODS: In the experiment, intoxication in rats was modeled with the fungicide Thiram® (dimethylcarbamothioylsulfanyl N,N-dimethylcarbamodithioate, 1.6 mg per animal of 200 g mass) for 28 days, which led to the development of environmental dysbiosis. The detected changes were subsequently corrected within 21 days with the probiotic Bifidumbacterin® (0.14 ml per animal of 200 g mass) and the symbiotic Acipol® (0.08 ml per animal of 200 g mass). The composition of the microflora of colon mucosa of rats was studied using a bacteriological method. The activity of antioxidant protection enzymes of colonocytes was studied by the content of superoxide dismutase and catalase. The condition of lipid peroxidation processes was assessed by the content of diene conjugates and malondialdehyde.
 RESULTS: In introduction of Thiram®, reduction of the amount of obligate and opportunistic representatives of colon microbiocenosis was noted, as well as intensification of lipid peroxidation processes in the conditions of weakening of antioxidant protection in colonocytes. Application of Bifidumbacterin® and Acipol® resulted in increase in the amount of obligate bacteria and decrease in the content of opportunists of the colon microbiota with the strongest effect of Acipol® on the values of the determined parameters. When studying changes in biochemical parameters in animal colonocytes, both drugs were found to contribute to the normalization of the content of malondialdehyde in the colon tissue, besides, Acipol® led to a decrease in the concentration of diene conjugates in colonocytes.
 CONCLUSION: Introduction of Thiram® in all the specified periods affected the qualitative and quantitative composition of the microbiota and biochemical parameters in the colonocytes of the animals. The use of Bifidumbacterin® and Acipol® preparations resulted in the restoration of the qualitative and quantitative composition of colonic microorganisms, and also had a positive effect on the content of lipid peroxidation products in colonocytes, but more efficient was found to be Acipol® symbiotic.
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