Abstract
Cyclic uniaxial load tests were performed on natural and chemically modified bovine pericardium which is used in the construction of heart valve substitutes. A template was employed to select specimens from the same sites in different pericardial sacs. When the pericardium was chemically modified by glutaraldehyde fixation as an entire sac the tissue showed increased extensibility after modification compared with the natural tissue. The undeformed stress-free length (gauge length) in both the natural and modified tissue was determined by a highly reproducible experimental method giving a coefficient of variation of less than 0.5%. Specimens excised from a natural pericardial sac demonstrated a significant increase ( p < 0.03) in length, 4.97 ± 3.49%, after a single load cycle, compared with controls placed in isotonic saline but not mechanically tested. The test pieces had returned to their orginal length by 8.5 h after the mechanical test. After fixation the same specimens decreased significantly in length ( p < 0.001) by 11.18 ± 4.28%. This shrinkage was not significantly different to that of the control specimens (11.09 ± 2.47%) which had not been tested. Uniaxial loading of these chemically modified strips demonstrated a similar increase in tissue extensibility compared to the natural tissue if the undeformed length of the test specimen after shrinkage was used as the gauge length. After mechanical conditioning the chemically modified tissue also demonstrated a significant increase ( p < 0.001) in stress-free length (5.35 ± 0.59% after 36 cycles, 8.92 ± 1.50% after 2085 cycles). These deformations were not permanent. The tissue had returned to its original length after 38 h in its normal buffered glutaraldehyde storage medium. On the basis of these observations, recommendations for the clarification and standardisation of gauge length definitions were made in natural, chemically modified and mechanically conditioned tissue.
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