Abstract
The transcription in vitro of the tryptophan (trp) operon of Escherichia coli was studied with a preparation consisting of purified RNA polymerase from E. coli and purified DNA from a trp transducing strain of ϕ80. The synthesis of trp mRNA was determined a) in a two-step RNA-DNA filter hybridization test (measurement of the amount of RNA which remains free after hybridization with ϕ80 DNA and hybridizes with ϕ80 trp DNA)
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