Abstract

The fate of 3H in mice given 3H-proline has been examined by amino acid and radiometric analyses of calvarial bone hydrolysates. The proline and hydroxyproline fractions contained 80% of the isotope, and 3H-hydroxyproline represented 25% of the total bone radioactivity. These findings indicate that bone matrix formation may be evaluated accurately in the calvaria by measuring the amount of isotope per mg of bone.

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