The specificity of induction of α-galactosidase from Saccharomyces carlsbergenensis

Abstract

A number of sugars and derivatives have been tested for their ability to induce the synthesis of α-galactosidase from Saccharomyces carlbergensis. Besides galactose and the substrates of the enzyme melibiose, raffinose and stachyose, D-galacturonic acid, L-arabinose, D-tagatose, methyl-α-D-galactoside, lactose and isopropyl-β-D-thiogalactoside were able to act as inducers. Of these, metyl-α-D-galactoside, lactose, isopropyl-β-D-thiogalactoside and L-arabinose have been shown to be gratuitous inducers with which kinetic studies of induction have been carried out. Lactose was the most efficient inducer, giving a maximal differential rate of synthesis of the enzyme of 110 mU/10 7 cells at a concentration of 190 mM, followed by L-arabinose (60 mU/10 7 cells at a concentration of 180 mM, followed by L-arabinose (60 mU/10 7 cells at 40 mM), isopropyl-β-D-thiogalactoside (43 mU/10 7 cells at 60 mM) and metyl-α-D-galactoside (25 mU/10 7 cells at 150 mM). The concentration of inducer required to obtain half-maximal induction was similar for lactose, L-arabinose and isopropyl-β-D-thiogalactoside and about 5-fold higher for methyl-α-D-galactoside. The property of the compounds to act as inducers was compared to their ability to interact with the enzyme and the results discussed in terms of the molecular structures which are recognized by the enzyme and by the induction machinery.