Abstract
The Asr gene is related to a number of functions, such as sugar regulation, stress induction, and fruit ripening in many plants, including tomato, potato, grape, pine, and rice. We used the promoter of the ci21A gene of potato, which belongs to the Asr family, to characterize the genetic expression of this gene in tomato and Arabidopsis. Particularly, there is known to be no Asr gene in Arabidopsis. In order to identify the spatial and temporal expression of the ci21A promoter, we used β-glucuronidase (GUS) as the reporter gene. The ci21A-GUS fusion gene was found to exhibit a very similar expression pattern in transgenic tomato and Arabidopsis. The distribution of GUS revealed that most of the expression appears in the sink organs of tomato and Arabidopsis. The expression pattern controlled by ci21A promoter helped us to clarify the role of the ASR protein in the developmental processes of plants. It also provided the possibility of applying this promoter in the expression of foreign genes in plants. The ci21A promoter could be suitable for various applications in different species.
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