Abstract
Drought-induced (Di19) proteins played important roles in plant growth, development, and abiotic stress responses. In the present study, a total of seven Di19 genes were identified in soybean. Each soybean Di19 gene showed specific responses to salt, drought, oxidative, and ABA stresses based on expression profiles. With a relatively higher transcript level among Di19 members under four stress treatments, GmDi19-5 was selected for detailed analysis. Inhibitor assays revealed that ABA inhibitor (Fluridone) or H2O2 inhibitor (DMTU) was involved in the drought- or salt-induced transcription of GmDi19-5. The GUS activity driven by the GmDi19-5 promoter was induced by salt, PEG, ABA, and MV treatments and tended to be accumulated in the vascular bundles and young leaves. A subcellular localization assay showed that GmDi19-5 protein localized in the nucleus. Further investigation showed that GmDi19-5 protein was involved in the interaction with GmLEA3.1. Overexpression of GmDi19-5 increased sensitivity of transgenic Arabidopsis plants to salt, drought, oxidative, and ABA stresses and regulated expression of several ABA/stress-associated genes. This present investigation showed that GmDi19-5 functioned as a negative factor under abiotic stresses and was involved in ABA and SOS signaling pathway by altering transcription of stress-associated genes.
Highlights
Abiotic stress may occur at any stage of plant development and often several types of stresses occur simultaneously (Rizhsky et al, 2004)
They contained conserved two conserved C2H2 zinc finger domains which were consistent with Arabidopsis Di19 homologs (Supplementary Dataset S1)
Based on suggested Arabidopsis Di19 nomenclature, each gene was named with a two-letter code corresponding to G. max (Gm), followed the family designation (Di19), and a number (Table 1)
Summary
Abiotic stress may occur at any stage of plant development and often several types of stresses occur simultaneously (Rizhsky et al, 2004). Zinc finger proteins are one of the major families of eukaryotic transcription factors (Klug and Schwabe, 1995; Takatsuji, 1999; Englbrecht et al, 2004). Within this family, the Cys2/His2-type (C2H2) zinc finger proteins contain one or more tandem of C2H2 zinc finger motifs, one of the best-characterized DNA-binding motifs. Drought-induced (Di19) proteins are zinc finger transcription factors that play important roles in development, growth, and response to stress (Milla et al, 2006; Li et al, 2010a,b; Liu et al, 2013b; Qin et al, 2014). Di19 proteins are known in cotton (Li et al, 2010a) and wheat (Li et al, 2010b)
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