The Sorangium Cellulosum Immobilized by Porous Ceramic Fermentation for Epothilone Preparation

Abstract

The Epothilone,produced by Sorangium cellulosum,has great medicinal value,however,the change of growth form on Sorangium cellulosum has limited the mass produce of Epothilone in the liquid circumstance. the porous structure of porous ceramic can provide a solid attachment and growth surface,then improve the production of Epothilone. The diatomite-based porous ceramics was made from diatomite by adding pore-making agent method. After optimized the preparation and modified conditions,it is found that best prescription contains 2. 5% of 30 mesh pore-forming agent of wood powder( mass fraction),the forming pressure at 7MPa,the performance of porous ceramics are pore diameter 5 μm,specific surface 23. 55 m2/ g,porosity 32% and mechanical strength 10. 2MPa. The adsoption capacity of porous ceramics,which is modified by 1. 5mol / L FeCl3,is 36. 8mg/g. The optimal immobilized fermentation condition is:the liquid volum 45mL in the 300mL flask,the porous ceramics 2. 7g modified by 1. 5mol / L FeCl3,inoculum 10%,temperature 30℃,shaking speed 220r / min,initial pH 7. 5 and fermentation time 8d. the Epothilone production reached 90. 2mg / L,which is increased 4 times.