Abstract
SUMMARY A consistently different phenotype was observed in Nicotiana benthamiana and Nicotiana tabacum cv. White Burley plants infected by the Brazilian Tomato spotted wilt virus (TSWV) isolate Br20 and the two Italian isolates p272 and p105/43.14. Based on the phenotype of experimentally obtained reassortants carrying the small (S) genomic segment of p272 or p105/43.14 and the medium (M) and large (L) segments of Br20, we concluded that the small segment of Br20 is required for eliciting lethal necrosis in N. benthamiana and local necrotic lesions in N. tabacum. To evaluate whether the Br20 nucleocapsid (N) or the non structural (NSs) protein encoded by the S segment were able to cause the distinctive symptoms, either alone or in combination, the three allelic variants (Br20, p272 and p105/43.14) of each gene were transiently expressed in planta through agroinfiltration. All the alleles of the N and NSs proteins accumulated abundantly in infiltrated N. benthamiana and N. tabacum leaves. No specific necrotic reaction was observed in any of the single or combined agro-infiltrations of the Br20 alleles, suggesting that the S-encoded proteins of isolate Br20 are not sufficient to elicit the specific symptomatic response and that the co-involvement of other regions of the genome might be necessary.
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