Abstract
Aims The study aims to explore the effects of the single-nucleotide polymorphism of miR-27a and its expression in Helicobacter pylori (H. pylori)-related diseases and the relationship between gastric pathology and traditional Chinese medicine (TCM). Methods Subjects were classified into six histopathological groups and five TCM syndrome groups. All specimens underwent H. pylori detection through rapid urease test and methylene blue staining. Histopathological characteristics were observed by hematoxylin-eosin. The expression of miR-27a and its genotype were, respectively, detected by Quantitative Real-Time PCR and direct sequencing. Results H. pylori promoted the malignant evolution of gastric mucosa and were involved in the formation of TCM syndrome. In H. pylori-positive patients, the frequency of miR-27a CT genotype at the rs895819 locus and its expression in the gastric cancer group were higher than those in other pathological groups. TCM syndrome had a close relationship with histopathological changes, and patients with spleen-qi deficiency syndrome had a higher risk of gastric cancer than other syndromes, regardless of H. pylori infection. Conclusion The C allele at miR-27a rs895819 locus may be an oncogene in gastric cancer. High levels of miR-27a could play an important role in gastric malignant evolution, especially cancerization. There is a certain connection between TCM syndrome and pathological changes of the gastric mucosa to some extent, where patients with SQD syndrome had a higher risk of GC.
Highlights
Gastric cancer (GC) is the fifth most common cancer and the third most common cause of cancer death worldwide, especially in Eastern Asian and Eastern European countries [1–4]
Chronic uncontrollable inflammation caused by H. pylori is the first step before carcinogenesis, which develops into atrophy, intestinal metaplasia (IM), dysplasia, and eventually cancer [5–7]. e various lesions caused by H. pylori, covering the gastric mucosa from benign to malignant, are collectively referred to as H. pylori-related gastric disease (HPGD). e mechanism of HPGD is complicated and is related to the inflammation and immune response of gastric mucosa
In the traditional Chinese medicine (TCM) syndrome group, both the spleen-stomach dampness-heat (SSDH) and liver-stomach disharmony (LSD) groups had higher H. pylori infection rate than that in the NON and spleen-qi deficiency (SQD) groups, while it was higher in the SQD group than the NON group
Summary
Gastric cancer (GC) is the fifth most common cancer and the third most common cause of cancer death worldwide, especially in Eastern Asian and Eastern European countries [1–4]. E mechanism of HPGD is complicated and is related to the inflammation and immune response of gastric mucosa. In this process, the activation of related inflammatory pathways and the release of various inflammatory factors are the microscopic manifestations of gastric mucosal lesions. E TCM syndromes are a macroscopic manifestation of the pathological changes in gastric mucosa, which can reflect the pathological process of the gastric mucosa to a certain extent and can play a warning role in the evolution of gastric cancer [20–22]. Our study detected the SNPs of miR-27a rs895819 and the miR-27a expression in different stages of HPGD to explore the microscopic differences of TCM syndromes and interpret the mechanism of pathological changes in gastric mucosa from multiple perspectives and can provide an early warning basis for the clinic
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