Abstract

AbstractMany techniques are currently available that allow the isolation of DNA (1–7) or RNA (8–23), but such methods allow only the purification of one type of nucleic acid at the expense of the other. Frequently, when cellular material is limiting, it is desirable to isolate both RNA and DNA from the same source. Such is the case for biopsy specimens, primary cell lines, or manipulated embryonic stem cells.KeywordsIsoamyl AlcoholGuanidinium ThiocyanatePrimary Cell LineExcess EthanolSterile PolypropyleneThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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