Abstract
A rapid, simple and precise method by HPLC (high performance liquid chromatography) has been developed for simultaneous determination of water-soluble vitamins as thiamine(B 1), nicotinamide(B 3), panthotenic(B 5), pyridoxine(B 6) and biotin(B 8) in gum of Acacia nilotica using enzymatic hydrolysis. The method uses a C 18 column (4.6×150 mm, 5µm). Mobile phase such as methanol 0.1M, sodium dihydrogen phosphate (pH = 2.5), (10:90 v/v) is found most suitable for rapid separation and identification of this water –soluble vitamins. Good linearity was observed between the concentration of analytes and peak area (r = 0.9999). Each vitamin was quantitatively determined at its maximum wavelength. Recovery percentages ranged from 97% to 99%.
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