The significance of detection of nitrites in oral fluid of healthy people.

Abstract

The nitric oxide is a signal molecule in human organism characterized by a wide spectrum of biological effects. The exogenous nitric oxide is formed from food nitrates received with such green leafy vegetables as spinach, parsley, sorrel and also beet, cucumbers and tomatoes. The bacteria in oral cavity metabolize received with food nitrates up to nitrites. The nitrites as some intermediate metabolites of nitric oxide sufficiently exact reflect concentration of nitric oxide. The purpose of study is to examine analytical dependence and efficiency of express-device for detecting nitrites in saliva. The article presents the results of confirmation of testsystem for half-quantitative detection of content of nitrites in saliva. The device represents a hollow tube with a sensorial element within functioning by the principle of "dry chemistry". The concentration of nitrites in oral cavity was measured in 100 healthy people of both genders aged from 16 to 45 years. In case of consuming vegetables every day or 3-5 times a week higher levels of nitrite-anions (14,9-15,7 mg/l) are registered than in case of consuming vegetables and juices 1-2 times a week and rarely (9,9 mg/l). The concentrations of nitrites in saliva both in cases of regular training and low-activity life-style are within the limits of standards (11,9-14,9 mg/l). The average level of nitrites in saliva of participants of experiment with normal pressure made up to 16.5 mg/l that is significantly higher than in individuals from groups with increased and decreased arterial pressure (10,2 and 10,4 mg/l correspondingly).