Abstract
Procyanidins are plant polyphenolic compounds reported to play a beneficial role in inflammatory bowel disease which is usually accompanied with a reduced activity of the Na+/K+ ATPase. Thus, a correlation between procyanidins and the ATPase was suspected and was examined in this work. The activity of the ATPase was assayed by measuring the amount of inorganic phosphate liberated in presence and absence of ouabain. Caco-2 cells treated with Procyanidin B2 exerted a stimulatory effect on the Na+/K+ ATPase which disappeared in the presence of inhibitors of PI3K, PKC, PKA, and COX enzymes. Exogenous PGE2 stimulated the ATPase via EP4 receptors and PKA. PKC was found to be downstream PI3K and upstream PGE2 while p38MAPK had a modulatory role but was not along the signaling pathway. It was concluded that procyanidin B2 acts via PI3K, activating PKC and leading to PGE2 release. PGE2 then stimulates the ATPase via EP4 and PKA.
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