The short-term rinsing of airways by N-acetylcysteine helps expectoration: The mechanism of sodium and chloride transport

Abstract

N-acetyl-L-cysteine (NAC) mucolytic and antioxidant role is well known, but the effect on epithelial ion transport has not been yet described. The aim of the study was to evaluate the short-term and prolonged influence of NAC on ion transport in the epithelium. The experiment was performed on 108 fragments of rabbit tracheae. Fragments were divided into four groups: inhibited sodium (I) and chloride (II) transport, NAC with inhibited sodium (III) and NAC with inhibited chloride (IV) transport. The changes in electrophysiological parameters were measured in stationary conditions and during mechanical-chemical stimulation after immediate (15 s) and prolonged (60 min) N-acetylcysteine administration on the tissue. Each 15-second stimulation caused repeatable changes in the electric potential of the tissue. In trachea fragments with blocked chloride ion transport, significantly lower (P <0.0001) values of electric potential following prolonged NAC effect were observed when compared to short-term NAC-stimulation. The values of resistance were constant during experiments, which reflects the vitality of the tissue. Short-term NAC administration influences sodium ion transport, which is not observed in a prolonged stimulation. The use of the NAC solution to rinse the airways is of great clinical importance due to the short and intense contact with the epithelium.