The shoot growth and anatomical structure of Protocorm Like Body of Grammatophyllum scriptum (Lindl.) Bl. after kinetin and NAA application

Abstract

PLB of Grammatophyllum scriptum (Lindl.) Bl. was used as explants. The aim of this research was to know the influence of kinetin and Naphthalene Acetic Acid (NAA) application on PLB differentiation and shoot growth by in vitro culture. PLB were placed on medium containing basal salt Knudson C (KC). Research device the used is Completely Random Design (CRD) with six treatments, every treatment with five restating. Kinetin were used in combination with NAA ((K0N0: kinetin 0 mg/l and NAA 0 mg/l (control), K1N0: kinetin 1 mg/l and NAA 0 mg/l, K2N1: kinetin 2 mg/l and NAA 1 mg/l, K3N2: kinetin 3 mg/l and NAA 2 mg/l, K4N3: kinetin 4 mg/l and 3 mg/l, K5N4: kinetin 5 mg/l and NAA 4 mg/l) to shoot induction. The data of shoot induction was examined at week 2, 4, 6, 8, and 10. The parameter examined is amount of shoot formation, anatomy structure of PLB, shoot height, browning of shoot, and root formation. Analysis of Variance (ANOVA) was done to know the influence of kinetin and Naphthalene Acetic Acid (NAA) application on shoot formation and when by Duncan Multiple Range Test (DMRT) on 5% significant rate to find out the real distinction between the treatments. The result show that the used of kinetin 1 mg/l and NAA 0 mg/l was stimulating differentiation of PLB G. scriptum produced shoot apical and primordial leave at third week. The used of kinetin 5 mg/l and NAA 4 mg/l produced the most shoots, the highest shoot height, and root formation.