Abstract
Suspensions of syngeneic sarcoma cells were injected into the Peyer's patches of rats from which the mesenteric nodes had been removed. By later cannulating the thoracic duct of such rats it was possible to collect peripheral intestinal lymph that had come directly from the tumour bearing area without being filtered through a regional node. The number of viable tumour cells in the lymph coming from the tumours was monitored by culturing the whole lymph cells in a limiting dilution assay. The tumours grew to a diameter of approximately 1 cm in 25 days and during this time tumour cells were present in the lymph at a ratio of approximately 1 tumour cell per 10(5) lymph cells. In euthymic rats this number declined as the immune response developed. In athymic rats the number increased by approximately 10 fold during the experiments. It was concluded that the shedding of viable cells parallels the linear, not the volumetric dimensions of the tumour.
Highlights
Male hooded rats weighing 200-250 g were subjected to mesenteric lymphadenectomy; 6-8 weeks later their abdomens were re-opened and suspensions of syngeneic sarcoma cells were injected into each of the 6-8 major Peyer's patches in the small intestine
Lymph was collected over periods of 24hr under sterile conditions, and the number of viable tumour cells in each collection was determined by culturing the washed lymph cells in a limiting dilution assay
In 5 control rats with intact mesenteric nodes no tumour cells were detected in the thoracic duct lymph even though the original tumours in the Peyer's patches grew to over 1 cm in diameter, and viable tumour cells could be recovered from the mesenteric nodes
Summary
Male hooded rats weighing 200-250 g were subjected to mesenteric lymphadenectomy; 6-8 weeks later their abdomens were re-opened and suspensions of syngeneic sarcoma cells were injected into each of the 6-8 major Peyer's patches in the small intestine. From 1-20 days thereafter each rat was provided with a cannula in the cysterna chyli and placed in a Bollman cage so that thoracic duct (i.e. mainly intestinal) lymph could be collected quantitatively. Similar experiments were carried out on a control group of intact rats, i.e. rats which had not had their mesenteric nodes removed by prior surgery. In order to take into account the effects of the specific immune responses to the tumour, which must be presumed to have developed during the course of the experiment, an identical series of experiments was carried out on athymic (nude) rats
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