THE SEVERITY OF ENDOGENOUS INTOXICATION AND OXIDATIVE STRESS IN THE BLOOD OF WORKERS IN CONTACT WITH GLYCINE DERIVATIVES

Abstract

Introduction. Currently, the search for markers of the health status of workers in agro-industrial complexes in contact with pesticides continues. The aim of this study was the assessment of the effect of glyphosate isopropylamine salt on the health of workers in agroindustrial complexes of the Ryazan region according to the severity of endogenous intoxication and oxidative stress in the body. Materials and Methods. Plasma and erythrocytes of peripheral blood of workers were the clinical material for the study. Blood was taken in 87 workers contacted with glyphosate-containing pesticides. As a control group, 20 clinically healthy individuals of the same age category who did not have contact with the pesticides were selected to be studied. The severity of endogenous intoxication was determined by the level of substances of low and medium molecular weight in the plasma and red blood cells. The severity of oxidative stress was determined by the content of the products of oxidative modification of proteins by the method of R.L. Levine modified by E.E. Dubinina. Results. Statistically significant changes in the severity of endogenous intoxication in the blood plasma and erythrocytes of the study groups and the control group of individuals among machine operators and workers who come into contact with pesticides to a lesser extent were obtained. A statistically significant increase in the level of carbonyl derivatives compared with the control among machine operators who have greater contact with glyphosate-containing pesticides according to the time sheets has been revealed. The level of secondary carbonyl derivatives in machine operators was higher than that of warehouse workers and was statistically significant. Conclusion. A statistically significant increase in the level of secondary carbonyl derivatives among machine operators as compared with other workers is a late marker of oxidative stress, which indicates to a depletion of the reserve forces of the body. This may be due to longer contact with pesticides based on glyphosate machine operators as compared to other workers in the agro-industrial complexes.