The severity of acute hypoxaemia determines distinct changes in intracortical and spinal neural circuits.

Abstract

The purpose of this study was to examine how two common methods of continuous hypoxaemia impact the activity of intracortical circuits responsible for inhibition and facilitation of motor output, and spinal excitability. Ten participants were exposed to 2h of hypoxaemia at 0.13 fraction of inspired oxygen ( clamping protocol) and 80% of peripheral capillary oxygen saturation ( clamping protocol) using a simulating altitude device on two visits separated by a week. Using transcranial magnetic and peripheral nerve stimulation, unconditioned motor evoked potential (MEP) area, short-interval intracortical inhibition (SICI) and intracortical facilitation (ICF), and F-wave persistence and area were assessed in the first dorsal interosseous (FDI) muscle before titration, after 1 and 2h of hypoxic exposure, and at reoxygenation. The clamping protocols resulted in differing reductions in by 2h ( clamping protocol: 81.9±1.3%, clamping protocol: 90.6±2.5%). Although unconditioned MEP peak to peak amplitude and area did not differ between the protocols, SICI during clamping was significantly lower at 2h compared to clamping (P=0.011) and baseline (P<0.001), whereas ICF was higher throughout the clamping compared to clamping (P=0.005). Furthermore, a negative correlation between SICI and (rrm =-0.56, P=0.002) and a positive correlation between ICF and (rrm =0.69, P=0.001) were determined, where greater reductions in correlated with less inhibition and less facilitation of MEP responses. Although F-wave area progressively increased similarly throughout the protocols (P=0.037), persistence of responses was reduced at 2h and reoxygenation (P<0.01) during the clamping protocol compared to the clamping protocol. After 2h of hypoxic exposure, there is a reduction in the activity of intracortical circuits responsible for inhibiting motor output, as well as excitability of spinal motoneurones. However, these effects can be influenced by other physiological responses to hypoxia (i.e., hyperventilation and hypocapnia). NEW FINDINGS: What is the central question of this study? How do two common methods of acute hypoxic exposure influence the excitability of intracortical networks and spinal circuits responsible for motor output? What is the main finding and its importance? The excitability of spinal circuits and intracortical networks responsible for inhibition of motor output was reduced during severe acute exposure to hypoxia at 2 h, but this was not seen during less severe exposure. This provides insight into the potential cause of variance seen in motor evoked potential responses to transcranial magnetic stimulation (corticospinal excitability measures) when exposed to hypoxia.