Abstract

SummaryA simple, yet comprehensive comparative mapping approach on how to mine the current GenBank database in order to facilitate the gene and quantitative trait locus (QTL) mapping in livestock species is described. Using this method, the bovine cysteine‐ and histidine‐rich cytoplasmic protein (CYHR1) gene was characterized. The cDNA sequence of the bovine CYHR1 was determined using public expressed sequence tag (EST) puzzle solving and its genomic organization was identified by sequencing. A BLAST search against the human genome sequences indicated that the bovine CYHR1 gene tentatively contains at least three exons and two introns, spanning approximately 4 kb in the bovine genome. A total of 149 and 155 cows with high or low estimated breeding values (EBVs) in protein yield were genotyped on a G/A polymorphism in intron 1 by polymerase chain reaction restriction fragment length polymorphism (PCR‐RFLP) technique with digestion of restriction enzyme NlaIII. This substitution had no significant effects on protein yield in Canadian Holstein cattle, yet could be used as a marker for additional haplotype analysis.

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