Abstract
Glycoprotein material was isolated from bovine aorta by saline extraction and fractionally precipitated with (NH4)2SO4. The glycoprotein material which precipitated between 60–100% saturation of the salt was fractionated by electrophoresis in polyacrylamide gel. The glycoprotein with the fastest mobility was isolated from the gel after repeated electrophoresis as a homogeneous fraction. The glycoprotein contains 17.4 residues of galactose, 3.0 of glucose, 2.9 of fucose, 12.4 of hexosamine and 6.1 of sialic acid per mole of glycoprotein. Sialic acid was selectively removed from the glycoprotein by hydrolysis with neuraminidase, and step-wise hydrolysis of the glycoprotein with β-galactosidase, β-N-acetylglucosaminidase and β-glucosidase revealed certain sequences of mono-saccharides which included galactosyl-N-acetyl-glucosamine and fucosyl-glucosyl-galactose in the glycoprotein.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.