The separation of immunocyte subpopulations by use of various lectins.

Abstract

The use of lectins for the enrichment of various lymphocyte subpopulations was investigated. Bauhinia purpurea lectin (BPA) was found to be effective for the enrichment of B cells, and the B cells thus obtained were further fractionated with lentil lectin into subsets showing high and low responses to dextran sulfate. The ability of various lectins to selectively induce suppressor T cell activity or helper T cell activity was also examined. The suppressor T cells thus induced were enriched peanut lectin and, conversely, the helper T cells were enriched with Limulus polyhemus lectin. This method was applied to analysis of age-dependent changes in the levels of suppressor and helper T cells in autoimmune-prone mice. Cytotoxic T cells induced in an allogeneic mixed lymphocyte culture were enriched with Dolichos biflorus lectin (DBA). These cytotoxic T cells showed a specific killing effect in vitro. However, when spleen cells of tumor-bearing mice were fractionated by use of DBA, the DBA- cells mediated the regression of the tumors in vivo. BPA was also found to be effective for the enrichment of the interleukin-2-producing T cell subset and macrophage precursor cells. Using this technique, bone marrow cells of autoimmune-prone MRL/1 mice were found to be rich in macrophage precursor cells.