Abstract
The increased use and applicability of Cannabis and Cannabis-derived products has skyrocketed over the last 5 years. With more and more governing bodies moving toward medical and recreational legalization, the need for robust and reliable analytical testing methods is also growing. While many stationary phases and methods have been developed for this sort of analysis, chiral stationary phases (CSPs) are unique in this area; not only can they serve their traditional chiral separation role, but they can also be used to perform achiral separations. Given that mixtures of cannabinoids routinely contain enantiomers, diastereomers, and structural isomers, this offers an advantage over the strictly achiral-only analyses. This work presents the separation of a 10-cannabinoid mixture on several polysaccharide-based sub-2 µm CSPs with both normal-phase and reversed-phase ultra-high-performance liquid chromatography (UHPLC) conditions. Along with the separation of the mixture, appropriate single-peak identification was performed to determine the elution order and reported where applicable.
Highlights
Like many other naturally sourced materials, Cannabis contains a complex blend of identified and unidentified compounds ranging from major and minor cannabinoids, terpenes, derivatives of these compounds, and other commonly occurring plant-based compounds such as chlorophyll [1,2,3]
Applications have been present for the analysis and quantification of other major and minor cannabinoids, pesticide testing, and terpene/terpenoid analysis [6,7,8,9,10,11,12,13,14,15,16,17]
The screening of the 10-cannabinoid mixture was performed under normal-phase conThe screening of the 10-cannabinoid mixture was performed under normal-phase ditions of 90-10-0.1 = Hex-EtOH-TFA and 90-10-0.1 = Hex-IPA-TFA
Summary
Like many other naturally sourced materials, Cannabis contains a complex blend of identified and unidentified compounds ranging from major and minor cannabinoids, terpenes, derivatives of these compounds, and other commonly occurring plant-based compounds such as chlorophyll [1,2,3]. This can make the accurate analysis of Cannabis for the levels of tetrahydrocannabinol (THC), levels of other cannabinoids, and presence of pesticides (as examples) challenging and complicated. Applications have been present for the analysis and quantification of other major and minor cannabinoids, pesticide testing, and terpene/terpenoid analysis [6,7,8,9,10,11,12,13,14,15,16,17]
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