Abstract
The separation of a Penicillium fungal extract by two dimensional-multimodal thin layer chromatography and gradient high performance liquid chromatography is reported. The fungal extract was resolved on a cyano derivatized silica gel TLC plate using two dimensional development; first dimension in methylene chloride: hexane:acetic acid (9:10:1), taken out, dried, turned 90°, and developed in the second dimension in acetonitrile:methanol: water (40:37:32). Over 20 different spots were observed under UV radiation. Three different mobile phase gradients, acetonitrile, methanol, and tetrahydrofuran as primary organic solvents were used. Different selectivities resulted in each gradient. The best result was obtained using the acetonitrile gradient, 25% to 100% acetonitrile in 30 minutes. Also, it was found that using photo diode array detection in natural products research is a must when quantitation is required, since the mixture contains compounds of different absorptivities. The results show that changing the wave length of detection resulted in different peak heights (areas).
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