Abstract
In the prebiotic synthesis, it was found that the formation of adenosine and adenosine triphosphate from adenine and ribose by ultra-violet irradiation only took place in the presence of phosphoric acid or polyphosphate ester[1,2].Gagik G. Gurzadyan reported the damage to uraciland adenine-containing bases,nucleosides and nucleotides on irradiation at 193 and 254 nm [3]. We found that the photolysis of a series of bases,nucleosides and nucleotides were sharply enhanced by photoionization of orthophosphate under irradiation of medium pressure mercury arcs (MPMA) at 190-220 nm[4]. Cytosine in phosphate buffer system was selected to study the sensitization mechanism of phosphate effect. The main photochemical reaction product have been isolated by chromatography on a 3 cm × 30 cm column of Zerolit F and purified by recrystallization. The molecular formula of photoproduct by elemental analysis is C 4 N 3 H 6 0 5 P with characteristic absorption maxima at 215 nm and 285 nm and molar extinction coefficient 1.22 × 10 4 and 5.43 × 10 3 respectively. The infrared spectra and phosphorus nuclear magnetic resonance spectrum appeared to be consistent with mechanism indicated phosphate anion radical substitutes hydrogen atom to form cytosine hydrophosphate. Oxygen is necessary for sensitizing the reaction.
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