The Sensitivity of Leersia hexandra Sw. to Gamma-Ray Irradiation

Pritha Kartika Sukmasari,Wahyu Widoretno,Dian Siswanto

doi:10.21776/ub.jels.2021.011.01.02

Pritha Kartika Sukmasari, Wahyu Widoretno + Show 1 more

Open Access

https://doi.org/10.21776/ub.jels.2021.011.01.02

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Abstract

Gamma-ray irradiation as a physical mutagen has high penetrating power. Therefore, it is most often used to increase genetic variability or produce new mutant plants. This research was conducted to obtain the lethal dose of gamma-rays in Leersia hexandra plants. The used plant part was a single node stolon which had a length of 10 cm with the node in the middle of the stolon. The irradiation doses given were 0, 25, 50, 75, 100 Gy. Stolons were inserted into plastic clips and irradiated using a Cobalt-60 gamma irradiation source at the Center for Irradiation and Radioisotope Applications (PAIR), National Nuclear Energy Agency (BATAN) Jakarta. Gamma-irradiation has a significant effect on inhibiting shoot growth. The growth of irradiated Leersia hexandra with the best number of plants and the highest shoots was obtained at a dose of 25 Gy and decreased with increasing irradiation dose. The lethal dose (LD 50 ) was determined eight days after irradiation using CurveExpert 1.4 software. Leersia hexandra plants that can sprout and regenerate followed the linear equation y = 1.02 - 7.5x with LD 50 at 68.85 Gy and LD 20 at 29.36 Gy. Keywords: gamma irradiation, genetic variation, Leersia hexandra , lethal dose, mutant plant.

Highlights

Forage is one of the determining factors in the development of the ruminant livestock business
Mutant plants obtained from gamma irradiation are generally at or slightly below the LD50 value [10]
The same results were shown by Zanzibar and Dede's research, that the highest irradiation dose of 100 Gy resulted in plants growing at the lowest as about 5.33% on Magnolia champaca [16]

Summary

INTRODUCTION

Forage is one of the determining factors in the development of the ruminant livestock business. The natural swamp plants generally have limitations in their nutritional value, such as their protein content. Natural grass has a scarce protein content which is around 4% [2] Due to overcome this limited nutritional value, a plant engineering effort is needed. Mutant plants obtained from gamma irradiation are generally at or slightly below the LD50 value [10]. The used plant material was L. hexandra stolon obtained from the experimental garden collection of the Beef Cattle Research Station. The stolons as a control that were not irradiated by gamma rays remained in the plastic clip to keep the stolons moist until implanted. The preliminary research results showed that the number of shoots growing from 100 stolons without irradiation was 69 shoots (control). Individuals with height in each of these clusters were counted [13]

AND DISCUSSION Shoot growth

Findings

CONCLUSION