The Selective Characterization of Nonthermal Atmospheric Pressure Plasma Jet on Treatment of Human Breast Cancer and Normal Cells

Abstract

The aim of this paper is to investigate the effect of nonthermal atmospheric pressure plasma jet on human breast cancer and normal cells. High voltage, dc-pulsed power supply was used to generate low temperature helium plasma in atmospheric pressure. In this paper, cancer and normal cells were exposed to plasma during four time steps. For further exploration, Doxorubicin as common chemotherapy drug was employed to compare the efficacy of plasma treatment with convectional cancer therapy. Also, the Caspase-Glo 3/7 assay was obtained to reveal the death process of cancer and normal cells. The results of MTT and apoptosis assay showed that the plasma treatment has drastically reduced the viability of breast cancer cells, while it has no significant damage to the normal cells. In addition, after plasma treatment three different zones were formed in the plate, which will represent the detachment of cells from plate surface. Moreover, adding 5% oxygen to the helium plasma will lead to enhancement of cancer cells viability reduction. In addition, the outcome of this paper has verified that the plasma treatment successfully overcomes drug treatment in inhibition of the cancer cells viability, while decreases the adverse effect of drug treatment.