THE SELECTION OF RESISTANT VARIANTS OF OPPORTUNISTIC MICROORGANISMS USING AETHONIUM IN CLINICS FOR 40 YEARS

Abstract

vitis. Bukovinian Medical Herald. Т.21, № 3 (83). P. 83-90 THE SELECTION OF RESISTANT VARIANTS OF OPPORTUNISTIC MICROORGANISMS USING AETHONIUM IN CLINICS FOR 40 YEARS L.I. Sydorchuk, I.P. Burdeniuk, S.E. Deineka, K.I. Yakovets, I.Y. Sydorchuk, V.I. Burdeniuk1, K.G. Tashchuk Objective: To establish the minimum bacteriostatic and bactericidal concentration of aethonium in relation to conditionally pathogenic microorganisms isolated and identified from the upper respiratory tract of children aged 11-13 years with chronic catarrhal gingivitis with a comorbid state (type I diabetes mellitus) in a comparative sense with sensitivity to aetonium of reference and clinical strains of conditionally pathogenic microorganisms isolated in 1974-1976. Materials and methods. Primary materials of 1974-1976 years of the sensitivity of conditionally pathogenic microorganisms to aethonium were used from dissertational works of co-authors (I.P. Burdeniuk, I.Y. Sydorchuk) for comparison of data obtained in 2014-2016. Microbiological examinations were performed in 30 pupils aged 11-13 years with catarrhal gingivitis with type I diabetes mellitus. The sampling of the material for microbiological examination was carried out with sterile tampons on the oral mucosa, following by seeding the sectors of solid nutrient media optimal for each taxon to obtain pure cultures. To determine the sensitivity of microbes to aethonium, the method of serial two-fold dilutions in a liquid nutrient medium was used. Results. It is shown that the sensitivity of the reference strains of microorganisms for 40 years of storage under laboratory conditions in a semi-solid nutrient medium remains practically unchanged. The bactericidal concentration of aethonium relative to S. faecalis ATCC 29212 increased by 1,96 %. It was shown that the bacteriostatic and bactericidal activity of aethonium decreased over 40 years relative to clinical strains and this decrease (resistance growth) depends on microorganisms, their morphological, tinctorial and other properties. Grampositive and gram-negative clinical strains of microorganisms have decreased sensitivity from 1,21 to 2,56 times. Conclusions. The development of resistant variants of microorganisms proceeds by gradual selection with dissemination in clinics. Before using aethonium, it is necessary to isolate and identify conditionally pathogenic microbes and to establish the bacteriostatic and bactericidal concentration of aethonium with the subsequent solution of the question of its possible effective use.