Abstract
In a study to optimize the use of the bar gene as a selectable marker gene in plant transformation, the selection mechanism of phosphinothricin in vitro was studied. It was found that tissues with a high anabolic activity were more sensitive to the toxicity of ammonium than tissues with a low anabolic activity. However, tissues with a low anabolic and high catabolic activity were more sensitive to glutamine deprivation. The metabolic (anabolic and catabolic) activity of callusing hypocotyl explants of Brassica napus L. was influenced by the carbon source of the medium and the light condition. The expression of the bar gene regulated by the light-inducible promoter derived from the gene coding for the small subunit of ribulose-1,5-bisphosphate carboxylase-oxygenase was also influenced by both light condition and carbon source. Finally, a bioassay to screen for, and to quantify the resistance of expiants to, phosphinothricin is presented.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
