The secretion of the eggshell of Schistocerca gregaria: ultrastructure of the follicle cells during the termination of vitellogenesis and eggshell secretion.

Abstract

The secretion of the eggshell by the follicle cells in the desert locust, Schistocerca gregaria, was studied using the electron microscope. The 3 layers of the eggshell, the vitelline membrane, the endochorion, and the exochorion, are produced in sequence over a short period of about 30-36 h. The follicle cells contain little rough endoplasmic reticulum (RER) and small inconspicuous Golgi bodies during vitellogenesis. As eggshell secretion approaches there is an increase in the amount of RER and Golgi cisternae contain electron-dense product. At each stage of the 3-phase secretion cycle the follicle cells contain Golgi bodies and secretion vesicles with distinct morphology. The follicle cells increase in breadth and decrease in height between the beginning and end of eggshell secretion. The endochorion ridges arise at the junction between follicle cells and appear to be moulded by the microvilli formed at this position. In the ovary prior to ovulation, the eggshell consists of a thin (0.5 micrometer) electron-dense vitelline and an outer fibrillar exochorion layer, 20-30 micrometer thick. Further changes take place in the vitelline membrane and the endochorion after oviposition, and a layer of curly fibres, the extrachorion, is secreted in the oviduct.