The Secchi disk depth to water depth ratio affects morphological traits of submerged macrophytes: Development patterns and ecological implications

Abstract

Water clarity, represented by Secchi disk depth (SD), and water depth (WD) alter bottom light availability, and SD/WD is critical for morphological trait development of submerged macrophytes in freshwater ecosystems. However, the underlying mechanism and trait development patterns of submerged macrophytes to a decreasing SD/WD gradient remains largely unknown. Here, we performed a 42-day mesocosm experiment with the erect type submerged macrophyte, Hydrilla verticillata, along a decreasing SD/WD gradient to study the relationship of morphological trait development with light availability, to determine the critical SD/WD at which changes in the development of morphological traits occur, and to gain insights into the potential mechanism involved. The results indicate that most of the morphological traits, including biomass, relative growth rate, number of clonal propagules, and the root/shoot ratio decreased with a decrease in the SD/WD ratio. Conversely, plant height and shoot increment rate increased with a decrease in the SD/WD ratio. Principal component analysis indicated that the SD/WD ratio is critical in determining the growth, stability, and reproduction of H. verticillata, and that only SD/WD ratios ≥ 0.45 and ≥0.55 ensured growth ability and stability, respectively. Possible development patterns of functional traits in relation to SD/WD reduction were investigated, and patterns of key traits of H. verticillata were distinct from those of Vallisneria natans, indicating different strategies for the adaptation to conditions of decreasing light availability. These results highlight the role of adaptive changes in morphology, resource allocation and life strategies for the maintenance of growth, stability and resilience of submerged macrophytes in low light conditions. Our present study provides a basis from which we could enhance our understanding of the critical transition mechanisms involved in morphological trait development in response to bottom light availability.