Abstract
This survey’s aim was to investigate the seasonal effect of Fatty acid synthesis (FAS) expression on intramuscular fat (IMF) content in grazing Mongolian, Ujumqin and grain-fed Ujumqin sheep. Different skeletal muscles of sheep (biceps femoris, longissimus dorsi and triceps brachii) were sampled to measure IMF content and total RNA was extracted to determine FAS mRNA expression levels by real-time PCR. The result revealed that: (1) The difference in IMF content in the muscles of Mongolian sheep grazed in summer was observed to be much higher than sheep grazed in winter (p<0.01). Also there was significant difference in IMF content in the muscles of Ujumqin sheep grazed in summer than winter (p<0.05). The mRNA expression level of FAS in muscles of winter grazing Mongolian sheep was significantly higher than summer (p<0.05). The seasonal effects on IMF content in the muscles and it’s FAS mRNA gene expression was all negative correlated between the sheep breeds. Accordingly, grazing Mongolian sheep’s FAS gene expression level was very high negatively correlated (r=-0.964). This study suggested that grazing Mongolian and Ujumqin sheep able to store large amount of IMF under depends on seasonal effects.
Highlights
Mongolia has a long tradition of raising livestock
There have been some studies on the relationship between intramuscular fat (IMF) content and Fatty acid synthesis (FAS) mRNA expression level in the mammals such as pigs, rats, sheep and chicken [4, 13]
Fifty four 2 year old male sheep were taken for the experimental procedure at different seasons; 18 Mongolian sheep, 18 Ujumqin sheep and 18 grain-fed Ujumqin sheep (MAO’s ranch, Shilinhot, Inner Mongolia, China; FeedstuffsZhendgda feed) in Inner Mongolia)
Summary
Mongolia has a long tradition of raising livestock. Its pastoral production system dates back to at least thousand years. There are increasing concerns of society towards the consumption of animal products which have been produced and transformed in a sustainable manner. This trend influences consumer and their purchasing decision, in developed countries [12]. There have been some studies on the relationship between IMF content and FAS mRNA expression level in the mammals such as pigs, rats, sheep and chicken [4, 13]. We reared two breeds and 3 kind’s Mongolian sheep (grazing Mongolian, Ujumqin and grain fed Ujumqin) to be investigated the effects of seasonal changes on the IMF content and to study the expression of FAS mRNA in different skeletal muscles
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