The screening of bioactive compound of the green algae Halimeda macroloba (Decaisne, 1841) as an antioxidant agent from Banyak Island Aceh Singkil

Abstract

Banyak Island is one of the outest island in western Indonesia which high biodiversity particularly macroalgae. One of the macroalgae which have potential food and herbal drug was Halimeda macroloba. The aim of this study was to screen the bioactive compound of the H. macroloba as antioxidant source. The sampling of H. macroloba were located at the coast of Banyak Island, Aceh Singkil. The reseach stages including the chemical composition, extraction, phytochemical screening, the antioxidant assays (DPPH, CUPRAC and FRAP). The result showed the highest rendement content was obtained from ethanol extract as much as 2.32%. Subsequently, the extract of the H. macroloba detected phenol compound. The antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH) method were obtained IC50 121. 445 ± 1.03 mg/L, the n-hexane extract have IC50 181.945 ± 1.95 mg/L and ethyl acetate extract of IC50 228.67 mg/L. The antioxidant with ferric reducing antioxidant power (FRAP) method showed that the ethanol extract possesses the value of antioxidant capacity was 516.50±0.70 μmol trolox/g extract, ethyl acetate extract value was 482.00±1.41 μmol trolox/g extract and n-hexane extract was 323.50±0.70 μmol trolox/g extract. While, the CUPRAC (cupric ion reducing antioxidant capacity) with ethanol extract was 159.85 ±0.70 μmol trolox/g extract, ethyl acetate extract was 66.38±0.03 μmol trolox/g extract and n-hexane extract was 49.15 ±0.035 μmol trolox/g extract. The results from three methods of antioxidant showed the ethanol extract possesses high antioxidant capacity. Therefore, this research give fruitfull information regarding the potential green alga H. macroloba as new antioxidant sources for human health.