The roles of dental pulp cell derived exosomes in human umbilical vein endothelial cells migration

Abstract

Objective To investigate the effect of dental pulp cell derived exosomes (DPC-Exos) on human umbilical vein endothelial cells (HUVECs) migration in vitro. Methods Exosomes were isolated from the supernatant of DPCs cell culture by ultracentrifugation and characterized by transmission electron microscope (TEM) , western blotting. The effect of DPC-Exos on HUVECs migration was examined by wounding healing assay and transwell assay in vitro. Two independent samples t test was used to statistically analyze the migration rate and number of migration cells by SPSS 20.0 software. Results The bi-layer membrane and saucer-like appearance of DPC-Exos were examined by transmission electron microscopy (TEM) . Western blotting revealed that CD63 was expressed in the DPC-Exos. Wound-healing assay showed significantly reduced migration ability by 26% in HUVECs after treated with DPC-Exos (t= 6.534, P<0.001) . Transwell assay results indicated that DPC-Exos treatment could significantly reduced migration ability of HUVECs by 32% (t= 5.846, P<0.001) . Conclusion DPC-Exos could significantly suppress the migration of HUVECs. Key words: Dental pulp; Exosomes; Cell migration assays; Angiogenesis