The role of WW domain-containing oxidoreductase in lymphocyte development

Abstract

Event Abstract Back to Event The role of WW domain-containing oxidoreductase in lymphocyte development Jui Yen Lo1 and Li-Jin Hsu2* 1 National Cheng Kung University, Institute of Basic Medical Sciences, Taiwan 2 National Cheng Kung University, Department of Medical Laboratory Science and Biotechnology, Taiwan WW domain-containing oxidoreductase (WOX1) is a newly isolated tumor suppressor protein. Downregulation of WOX1 protein expression has been found in many types of human cancers. Previous studies have suggested that WOX1 is involved in many signaling pathways that regulate cell apoptosis. Ectopic WOX1 increases tumor necrosis factor, staurosporine, UV or p53-induced cell death. Conversely, functional suppression of WOX1 by dominant negative mutants or siRNA protects cells from apoptosis. Although many studies have revealed a role of WOX1 in regulating cancer cell apoptosis, whether WOX1 is involved in lymphocyte development is unknown. To explore the functional role of WOX1 in vivo, we have recently generated whole-body knockout murine models by targeting exon 1 or exons 2-4 of the gene. All WOX1-deficient mice died by three weeks of age. Reduced body weights and marked atrophy of the spleen and thymus were observed in WOX1-deficient mice. Significantly reduced cell numbers of splenocytes and thymocytes were determined in WOX1-deficient mice, as compared with the wild-type and heterozygous mice. By TUNEL assay, massive thymocyte apoptosis was found in WOX1-deficient mice. Together, using gene knockout mice, our study clearly demonstrates that WOX1 is a key regulator in lymphocyte development. Keywords: tumor suppressor, gene knockout, Thymocytes, splenocytes, Apoptosis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Lo J and Hsu L (2013). The role of WW domain-containing oxidoreductase in lymphocyte development. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01053 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Li-Jin Hsu, National Cheng Kung University, Department of Medical Laboratory Science and Biotechnology, Tainan, 70101, Taiwan, hsu.lijin@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jui Yen Lo Li-Jin Hsu Google Jui Yen Lo Li-Jin Hsu Google Scholar Jui Yen Lo Li-Jin Hsu PubMed Jui Yen Lo Li-Jin Hsu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.